A Prevalence of Entamoeba Sp. in Children With Diarrhea
Keywords:
Entamoeba Histolytica Diarrhea, Interleukin (IL-8), Entamoeba SpAbstract
The objective of this study was to evaluate the prevalence of Entamoeba sp. in children with diarrhea who visited the Pediatric Teaching Hospital in Bagdad city and to estimate the level of serum IL-8 in patients. Patients and Methods: From the beginning of May 2022 to the end of November 2023, collected 112 fecal samples from children who had severe diarrhea or diarrhea with blood at the Pediatric Teaching Hospital in Bagdad city. A quantity of stool (especially mucous from it to increase the possibility of the parasite appearing) was taken and placed on a glass slide with a drop of glass physiological solution and a drop of iodine dye, and then examined under the microscope with a wooden stick and a drop of glass physiological solution and a drop of iodine dye, as well as the serum IL-8 levels were measured using the enzyme-linked immunosorbent assay (ELISA). Results: The percentage of samples evaluated by direct swab that was infected with the Entamoeba histolytica parasite The direct swab method was used to evaluate 112 fecal samples from children at the Pediatric Teaching Hospital, and the number of positive infections was 51 (45.53 %), So, a high infection ratio caused by Entamoeba sp. to ≥ 5 years old (43.5%) a parallel low infection ratio (23.5 %) at 10 ≤ years old , E.histolytica and E.dispar were highest (59 and 25 %) respectively in the current investigation, while E.coli infections were lowest (16%), as well as males reporting more E. histolytica cases (63%) than females (37 %).and Patients infected with Entamoeba histolytica had significantly higher IL-8 serum concentrations (35.600± 3.906) than controls (24.442± 2.411) (p<0.0001).Conclusion: High infection ratio caused by Entamoeba sp. to ≥ 5 years old, Infection rates with E. histolytica and E. dispar were highest, as well as males reported more E. histolytica cases than females, and Patients infected with Entamoeba histolytica had significantly higher IL-8 serum concentrations than controls.
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