An Extraction, Characterization and Evaluation of Antimicrobial Effectiveness of Raphanus Sativus Extracts
Keywords:
Enteric Bacteria, Raphanus Sativus, ExtractAbstract
ABSTRACT This study research aimed to prepare radish seeds extracts (aqueous and alcoholic) by maceration method and study the effect of both extracts on the enteric bacteria were lived in the intestine such as Escherichia coli bacteria and another types of Bacteria to show the effectiveness of both extracts. Chemically revealed to investigate the presence of active groups in both extracts containing tannins, glycosides, flavonoids, resins, saponins, and alkaloids which attributed the main effect to giving the effectiveness for anti-growth microorganisms. The biological effectiveness of the extracts was tested of E. coli bacteria and a number of bacterial species. The results showed that alcoholic extract was the stronger effectiveness than the aqueous extract and the standard gentamicin antibiotic 100% was the best concentration, the inhibition zone reached (32) mm for the E. coli bacteria compared to gentamicin antibiotic which inhibition zone reached just (17) mm. its showed also the effectiveness from another types of Bacteria (in the same concentration 100%), inhibition zone ranged between (28-32) mm compared to gentamicin that reached just (17) mm. it also showed the effectiveness for yeasts such as Candida albicans. Inhibition zone reached (21) mm compared to standard antibiotic nystatin which reached just (16) mm. As for the aqueous extract showed the effectiveness of concentration 100% about E. coli bacteria which inhibition zone reached (21) mm compared to gentamicin antibiotic which reached just (17) mm, also showed the effectiveness against Streptococcus mutans bacteria, the inhibition zone reached (20) mm compared to gentamicin that reached just (17) mm. the aqueous extract did not show any effect about types of another bacteria and yeasts.
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